Online Chemistry Labs | Learning Objectives


This experiment applies Gas Chromatography-Mass Spectrometry to the analysis of samples containing drug compounds. The student is provided with four solutions of known drugs, as well as a sample containing a mixture of all the drugs. The student will inject undiluted or diluted samples of the drugs into the GC to obtain chromatograms of the drugs along with corresponding tables showing the peak locations and signal intensities. Using volumetric equipment, the student can prepare a wide range of
dilutions of the known drugs in order to determine the detection limit of the method. Next the student is provided a synthetic sample of a sample taken from a human and will then determine the identity and concentration of the drug in that sample.

Analysis of Drug Samples by Gas Chromatography

Learning Objectives:

  1. Become familiar with the methodology and interpretation of gas chromatography.
  2. Be able to prepare dilutions of given drug samples for use in the analysis.
  3. Be able to compare undiluted and diluted drug samples, using peak locations and intensities to identify and quantify the drugs which are present.
  4. Be able to determine the detection limit for a given drug using this instrumentation.
  5. Be able to describe when the detection limit restricts the usefulness of a given result.
  6. Be able to answer questions about the use and limitations of this instrumental method.